Nature runes are runes that are used for transmutation spells. Members with a Runecrafting skill of 44 or greater can craft nature runes from Pure Essence at the Nature altar. Members with 91 or greater runecrafting always craft two nature runes per essence; those with between 44 and 90 runecrafting have a chance at crafting either one or two nature runes per pure essence, with the probability of two increasing with each runecrafting level. Crafting nature runes yields 9 runecrafting experience per essence (or 18 experience at the Ourania rune altar).

Nature runes are one of the five most traded items in the Grand Exchange because of the frequency of High Alchemy spells.